Product name
Rat IL-25 (Interleukin 25) ELISA Kit
Catalogue No.
ER1097
Detection method
Sandwich ELISA, Double Antibody
Application
IL-25 ELISA Kit allows for the in vitro quantitative determination of IL-25 concentrations in plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Storage
4 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of IL-25 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-25 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
92-98
95
EDTA plasma(n=5)
87-102
96
heparin plasma(n=5)
85-103
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-25 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
97-104%
95-104%
86-91%
86-98%
EDTA plasma(n=5)
83-94%
85-95%
82-94%
86-96%
heparin plasma(n=5)
81-99%
84-100%
93-99%
83-99%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only