Product name
Rat IL-1β (Interleukin 1 Beta) ELISA Kit
Catalogue No.
ER1094
Detection method
Sandwich ELISA, Double Antibody
Application
IL-1β ELISA Kit allows for the in vitro quantitative determination of IL-1β concentrations in plasma, tissue homogenates and other biological fluids.
Size
96T
Range
31.25-2000pg/ml
Sensitivity
< 18.75pg/ml
Storage
4 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of IL-1β and the recovery rates were calculated by comparing the measured value to the expected amount of IL-1β in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-105
95
EDTA plasma(n=5)
87-99
93
heparin plasma(n=5)
86-104
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-1β and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
85-101%
86-96%
85-105%
88-105%
EDTA plasma(n=5)
88-98%
83-99%
85-100%
86-101%
heparin plasma(n=5)
83-92%
84-99%
84-95%
87-97%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only