Product name
Rat IL-12 (Interleukin 12) ELISA Kit
Catalogue No.
ER1086
Detection method
Sandwich ELISA, Double Antibody
Application
IL-12 ELISA Kit allows for the in vitro quantitative determination of IL-12 concentrations in plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Storage
4 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of IL-12 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-12 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-103
92
EDTA plasma(n=5)
90-104
99
heparin plasma(n=5)
85-99
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-12 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
86-104%
92-103%
90-104%
87-100%
EDTA plasma(n=5)
85-98%
82-95%
84-98%
85-100%
heparin plasma(n=5)
84-96%
82-98%
84-100%
85-98%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only