Product name
Rat IL-10(Interleukin-10) ELISA Kit
Catalogue No.
ER0033
Detection method
Sandwich ELISA, Double Antibody
Application
IL-10 ELISA Kit allows for the in vitro quantitative determination of IL-10 concentrations in plasma, tissue homogenates and other biological fluids.
Size
96T
Range
31.25-2000pg/ml
Sensitivity
< 18.75pg/ml
Storage
4 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of IL-10 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-10 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
90-100
95
EDTA plasma(n=5)
91-103
96
heparin plasma(n=5)
86-101
91
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-10 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
88-98%
87-104%
85-100%
85-99%
EDTA plasma(n=5)
83-93%
82-92%
82-97%
82-101%
heparin plasma(n=5)
83-97%
82-91%
80-92%
81-99%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only