Specifications
Product name
Mouse IFN-β (Interferon Beta) ELISA Kit
Catalogue No.
EM1148
Alias
IFN-β ELISA Kit
Detection method
Sandwich ELISA, Double Antibody
Application
IFN-β ELISA Kit allows for the in vitro quantitative determination of IFN-β(Interferon Beta)/IFNB1/IFB/IFF/IFNB concentrations in plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Storage
4 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of IFN-β(Interferon Beta)/IFNB1/IFB/IFF/IFNB and the recovery rates were calculated by comparing the measured value to the expected amount of IFN-β(Interferon Beta)/IFNB1/IFB/IFF/IFNB in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-102
92
EDTA plasma(n=5)
90-97
93
heparin plasma(n=5)
85-104
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IFN-β(Interferon Beta)/IFNB1/IFB/IFF/IFNB and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
85-105%
86-104%
86-104%
88-96%
EDTA plasma(n=5)
86-98%
90-101%
83-101%
83-101%
heparin plasma(n=5)
81-94%
81-95%
80-96%
83-90%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only