Specifications
Product name
Mouse IFN-α-Ab (Interferon-α Antibody) ELISA Kit
Catalogue No.
EM1147
Alias
IFN-α-Ab ELISA Kit
Detection method
Sandwich ELISA, Double Antigen
Application
IFN-α-Ab ELISA Kit allows for the in vitro quantitative determination of IFN-α-Ab concentrations in plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Storage
4 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of IFN-α-Ab and the recovery rates were calculated by comparing the measured value to the expected amount of IFN-α-Ab in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-103
98
EDTA plasma(n=5)
87-103
94
heparin plasma(n=5)
89-104
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IFN-α-Ab and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
87-100%
91-99%
88-99%
86-100%
EDTA plasma(n=5)
82-92%
82-97%
88-96%
83-99%
heparin plasma(n=5)
81-96%
80-94%
89-100%
87-95%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only