Product name
Human IL-12 (Interleukin 12) ELISA Kit
Catalogue No.
EH0176
Alias
Interleukin 12 p70 ELISA Kit, CLMF p35 ELISA Kit, Cytotoxic lymphocyte maturation factor 35 kDa subunit ELISA Kit, IL-12 subunit p35 ELISA Kit, IL-12, subunit p35 ELISA Kit, interleukin 12, p35 ELISA Kit
Detection method
Sandwich ELISA, Double Antibody
Application
IL-12 ELISA Kit allows for the in vitro quantitative determination of IL-12 concentrations in plasma, tissue homogenates and other biological fluids.
Size
96T
Range
15.625-1000pg/ml
Sensitivity
< 9.375pg/ml
Storage
4 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of IL-12 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-12 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-105
97
EDTA plasma(n=5)
86-103
94
heparin plasma(n=5)
85-95
90
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-12 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
86-98%
85-103%
87-101%
93-105%
EDTA plasma(n=5)
83-92%
82-96%
91-97%
83-101%
heparin plasma(n=5)
83-97%
80-100%
83-95%
81-97%
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only