Name:
GAPDH Mouse Monoclonal
 
Description:
Mouse Monoclonal Antibody to GAPDH
Specificity:
Human, Mouse , Rat, Zebrafish
Applications:
ELISA, IHC, WB, IF, FC, IP
Immunogen:
glyceraldehyde-3-phosphate dehydrogenase (Calculated MW:36 kDa)
Host:
Mouse
Clonality:
Monoclonal
Isotype:
IgG1
Conjugate:
Unconjugated
Purity:
Protein A+G purification (≥95% as determined by SDS-PAGE)
Product Form:
Liquid
Formulation:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)
Recommended dilution
WB: 1:5000-1:20000; IP: 1:3000-1:20000; IHC: 1:50-1:500; IF: 1:20-1:200
 
Background
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules(By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate(G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT(gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3′-UTR of diverse inflammatory mRNAs(such as ceruplasmin) and suppresses their translation. This antibody is a mouse monoclonal antibody raised against full length GAPDH of human origin. It can recognize the 36kda GAPDH protein in most cells/tissues.
 
Uniprot information:
Gene Name
GAPDH
Uniprot ID
P04406
 
Target:
Synonyms:
38 kDa BFA-dependent ADP-ribosylation substrate Antibody
aging associated gene 9 protein Antibody
Aging-associated gene 9 protein Antibody
BARS-38 Antibody
cb609 Antibody
EC 1.2.1.12 Antibody
Epididymis secretory sperm binding protein Li 162eP Antibody
G3PD Antibody
G3PDH Antibody
G3P_HUMAN Antibody
GAPD Antibody
Glyceraldehyde 3 phosphate dehydrogenase Antibody
Glyceraldehyde-3-phosphate dehydrogenase Antibody
HEL S 162eP Antibody
HEL-S-162eP Antibody
KNC-NDS6 Antibody
MGC102544 Antibody
MGC102546 Antibody
MGC103190 Antibody
MGC103191 Antibody
MGC105239 Antibody
MGC127711 Antibody
MGC88685 Antibody
OCAS, p38 component Antibody
OCT1 coactivator in S phase, 38-KD component Antibody
peptidyl cysteine S nitrosylase GAPDH Antibody
Peptidyl-cysteine S-nitrosylase GAPDH Antibody
wu:fb33a10 Antibody
 
Research Areas:
Neuroscience → Neurology process → Neurodegenerative disease → Huntington's disease
Neuroscience → Neurology process → Neurodegenerative disease → Alzheimer's disease → Other
Isotype/Loading Controls → Loading Controls → GAPDH
Signal Transduction → Metabolism → Energy Metabolism
Cancer → Cancer Metabolism → Metabolic signaling pathway → Metabolism of carbohydrates
Metabolism → Pathways and Processes → Metabolic signaling pathways → Carbohydrate metabolism
Metabolism → Pathways and Processes → Metabolic signaling pathways → Energy transfer pathways → Energy Metabolism
Metabolism → Types of disease → Neurodegenerative disease
 
Please Note: GAPDH Mouse Monoclonal is for research use only. It is not intended for diagnostic or therapeutic use.